Determining Gene Location on Chromosomes of Bombyx mori Using Fluorescence in situ Hybridization (FISH)
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Single-gene detection and karyotyping using small-target fluorescence in situ hybridization on maize somatic chromosomes.
Combined with a system for identifying each of the chromosomes in a genome, visualizing the location of individual genetic loci by fluorescence in situ hybridization (FISH) would aid in assembling physical and genetic maps. Previously, large genomic clones have been successfully used as FISH probes onto somatic chromosomes but this approach is complicated in species with abundant repetitive ele...
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Fluorescence in situ hybridization (FISH) and its modification, polytene in situ hybridization (PISH) (1,2,4), have widespread application in the analysis of gene localization on interphase, metaphase and polytene chromosomes. However, available protocols use the indirect fluorescence approach, which requires the tedious and timeconsuming immunological detection step in which a fluorochrome-con...
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ژورنال
عنوان ژورنال: Applied Entomology and Zoology
سال: 1995
ISSN: 0003-6862,1347-605X
DOI: 10.1303/aez.30.225